Extended Data Fig. 1: Translocation kinetics of the Ig27V13P polyprotein.
From: Structural anisotropy results in mechano-directional transport of proteins across nuclear pores
(A) Nucleus-to-cytoplasm time course localization of the construct NLS-(Ig27V13P)X-mCherry-LEXY, being X the number of Ig27V13P (V13P) domains. (B) Import rate constants for the V13P polyproteins. Each construct was measured on at least three independent experiments, with n = 105 (V13P); n = 138 (V13P)2; n = 132 (V13P)3; n = 88 (V13P)4; n = 108 (V13P)5; n = 80 (V13P)6; n = 55 (V13P)7; n = 22 (V13P)8. Horizontal bars indicate the average import rate constant, and vertical bars the SEM.
