Extended Data Fig. 3: Translocation kinetics of the Ig27Y9P polyprotein.
From: Structural anisotropy results in mechano-directional transport of proteins across nuclear pores
(A) Nucleus-to-cytoplasm time course localization of the NLS-(Ig27Y9P)X-mCherry-LEXY construct, being X the number of Ig27Y9P (Y9P) domains. (B) Import rate constants for the Y9P polyproteins. Each construct was measured on at least three independent experiments, with n = 78 (Y9P); n = 127 (Y9P)2; n = 83 (Y9P)3; n = 58 (Y9P)4; n = 50 (Y9P)5; n = 56 (Y9P)6; n = 40 (Y9P)7; n = 27 (Y9P)8. Horizontal bars indicate the average import rate constant, and vertical bars the SEM.
