Extended Data Fig. 1: Experimental system.
From: Emergence of bidirectional cell laning from collective contact guidance
a: Schematic and notations of the grooved PDMS substrate. 0<d < 2.5 µm. b: Representative Scanning Electron Microscopy image of a grooved substrate c: Confocal fluorescence image of the fibronectin coating on the grooves prior to seeding the cells (fluorescently labeled fibronectin). This image is an average over 40 µm along the x-axis. d: Phase contrast image of single HBECs on grooved substrates. Cells typically span over 2 to 3 motifs laterally while they migrate in the groove direction. e: Phase contrast image of a confluent HBEC monolayer on a grooved substrate (20 h post-confluence). The double arrow represents the grooves direction. Note the convention for the x and y axis, as well as for velocity angle measurements. In B and D, groove depth=1.75 µm.
