Extended Data Fig. 2: Emergence of a laning pattern in HBEC monolayers on grooved substrates.
From: Emergence of bidirectional cell laning from collective contact guidance
a: Representative vx laning pattern on a grooved substrate (t = 20 h post-confluence, d = 1.75 μm). The direction of the grooves is given by the double arrow. b: Profile of the x component of the velocity vx along y. vx(y) is aperiodic and varies between two opposite values. The profile shown is a representative profile acquired along the vertical black line in panel A. c: Close-up of the vx(y) profile. Within a lane, the velocity profile is flat, corresponding to a plug flow. Between antiparallel lanes, vx changes sign over less than 50 μm (~ two cell sizes). d: The histograms of vx in the two boxes drawn in panel (A) are symmetrical and well-peaked at opposite values.
