Extended Data Fig. 3: Emergence of a laning pattern in HBEC monolayers on micro-patterned substrates.
From: Emergence of bidirectional cell laning from collective contact guidance
a: Compared to the grooved substrates, the micropatterned substrates have no topography but present an alternation of adhesive (fibronectin, cyan)/non-adhesive (PEG, pink) lines whose lateral dimensions are the same as the grooved substrates. b: vx cartography for an HBEC monolayer plated on a micropatterned substrate t = 20 h post-confluence. Cell flows self-organize in bilateral laning similar to the one obtained on grooved substrates (see Fig. 1j for comparison). On these micropatterned substrates, anisotropic friction does not result from grooves but from the contrast in cell-substrate adhesion between the fibronectin lines and PEG lines.
