Extended Data Fig. 1: Cell-type composition in crypts and the relocation of actomyosin.
From: Mechanochemical bistability of intestinal organoids enables robust morphogenesis
A. Cartoon to show the cell types in intestinal organoid. B. Left, representative images for the straining of Lgr5 in both bulged and budded crypts. Cell trace indicates all cells, H2B-iRFP marks cell nuclei and Lgr5-DTR-GFP marks intestinal stem cells. Right, Lgr5+ cell numbers are similar in bulged and budded crypts (sample number N = 18 (bulged) and N = 22 (budded), p = 0.22, two-tailed t-test). C-E. Representative images for the staining of Lyz (C), YAP (D) and Delta 1(E) in crypts. Paneth cell numbers are similar in bulged and budded crypts (sample number N = 42(bulged) and N = 49 (budded), p = 0.48, two-tailed t-test). F. Time-lapse recordings of basal crypt actomyosin relocation during lumen inflation. Red arrows point to the crypt basal surface with increasing Myh-9-GFP intensity. Scale bars: 50 µm. The box-plot elements (in B and C) show the 25% (Q1, lower bound), 50% (median, line within the box) and 75% (Q3, upper bound) quartiles, and the whiskers denote 1.5× the interquartile range (minimum = Q1–1.5\(\times\)(Q3–Q1), maximum = Q3\(+\)1.5\(\times\)(Q3–Q1)) with outliers (rhombuses).
