Extended Data Fig. 2: Quantification of tissue morphology and lumen volume.
From: Mechanochemical bistability of intestinal organoids enables robust morphogenesis
A. Quantification of crypt opening degree. Left, schematic to show data extraction for quantifying tissue opening angle (see Methods for details). Right, degree of crypt opening before and after lumen inflation, in both bulged (N = 11) and budded (N = 28) organoids. The box-plot elements show the 25% (Q1, lower bound), 50% (median, line within the box) and 75% (Q3, upper bound) quartiles, and the whiskers denote 1.5× the interquartile range (minimum = Q1–1.5\(\times\)(Q3–Q1), maximum = Q3\(+\)1.5\(\times\)(Q3–Q1)) with outliers (rhombuses). B, C. Quantification details of representative bulged and budded samples before and after lumen inflation. D. Quantification of lumen volumes before and after villus tissue breakage, in both bulged (top panels, N = 11) and budded (bottom panels, N = 9) organoids. Epithelial thickness in villus (absolute values and fold changes) can also infer the lumen volume changes: bulged samples show increased thickness of villus tissue after tissue breakage (top), indicating lumen volume decrease that is consistent with the volume measurement. Budded ones show the reverse trend (bottom). Scale bars: 50 µm (organoid) and 25 µm (magnification).
