Extended Data Fig. 10: NCED3 expression in CLE25-treated leaves of bam1-5 bam3-3 mutants, vascular development of wild-type, cle25 and bam1-5 bam3-3 mutants under control conditions and root growth phenotypes of cle25, nced3-2 and aba2-1 mutants under control conditions or long-term application of CLE25 peptide.
From: A small peptide modulates stomatal control via abscisic acid in long-distance signalling
a, NCED3 expression in the leaves of wild-type (Cont.) and bam1-5 bam3-3 mutants (n = 6 biological replicates) after application of CLE25 peptide to leaves. **P < 0.01 as analysed by one-way ANOVA followed by a Tukey’s post hoc test. b–e, Microscopy images of the leaf vasculature of wild-type (b; n = 6 biological replicates), cle25 #6 (c; n = 12 biological replicates), cle25 #10 (d; n = 12 biological replicates) and bam1-5 bam3-3 mutants (e; n = 12 biological replicates). Scale bars, 1 mm. f–i, Microscopy images of the protoxylem and metaxylem vessel formation of wild-type (f; n = 4 biological replicates), cle25 #6 (g; n = 3 biological replicates), cle25 #10 (h; n = 4 biological replicates) and bam1-5 bam3-3 mutants (i; n = 4 biological replicates). Scale bars, 50 μm. j–m, Cross section of primary root of wild-type (j; n = 9 biological replicates), cle25 #6 (k; n = 10 biological replicates), cle25 #10 (l; n = 10 biological replicates) and bam1-5 bam3-3 mutants (m; n = 8 biological replicates). Scale bars, 20 μm. n, Root length of wild-type and cle25 mutants from six to eleven days after germination, on germination medium agar plates (n = 16 biological replicates). o, Images represent 8-day-old or 10-day-old seedlings after germination of each genotype on germination medium agar plates (n = 4 biological replicates). Scale bars, 2 cm. p, Relative root length of wild-type, and nced3-2 and aba2-1 mutants from seven to eleven days after germination, on germination medium agar plate (n = 12 biological replicates). q, Images represent 8-day-old or 10-day-old seedlings after germination of each genotype on germination medium agar plates (n = 3 biological replicates). Scale bars, 2 cm. r, Relative root length of wild-type, and nced3-2 and aba2-1 mutants from seven to eleven days after germination, on germination medium agar plates containing 1 μM CLE25 peptide (n = 12 biological replicates). s, Images represent 8-day-old or 10-day-old seedlings after germination of each genotype on germination medium agar plates containing 1 μM CLE25 peptide (n = 3 biological replicates). Scale bars, 2 cm. Two-way ANOVA followed by a Tukey’s post hoc test indicated that there were no differences among each genotype (n, p, r).
