Extended Data Fig. 6: SRC-3 drives the purine synthesis program under conditions of active glycolysis.
From: Metabolic enzyme PFKFB4 activates transcriptional coactivator SRC-3 to drive breast cancer
a, MDA-MB231 cells stably expressing control shRNA, PFKFB4 shRNA and SRC-3 shRNA were fed with [6-13C]glucose. Ribulose/xylulose-5P (m + 1) labelling from [6-13C]glucose is shown. n = 3 biological cell samples. ***P = 0.00013, ****P = 0.000078, one-way ANOVA with Tukey’s multiple comparisons test. b, Genes involved in oxidative and non-oxidative PPP. n = 3 biological cell samples. *P = 0.0431, two-way ANOVA with Sidak’s multiple comparisons test. c, Genes involved in nucleotide synthesis. n = 3 biological cell samples. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA with Sidak’s multiple comparisons test. d, mRNA expression of the metabolic enzymes TKT, XDH and AMPD1 in MDA-MB-231 cells transduced with adenovirus expressing GFP (control) and PFKFB4 cultured in the presence of 5 mM, 15 mM or 25 mM glucose. n = 3 biological cell samples. **P < 0.01, ***P < 0.001, ****P = 0.0001, two-way ANOVA with Dunnett’s multiple comparisons test. e, f, MDA-MB231 cells stably expressing control shRNA, PFKFB4 shRNA and SRC-3 shRNA were fed with [6-13C]glucose. Seduheptulose-7P (m + 1) (e) and erythrose-4P (f) labelling from [6-13C]glucose are shown. n = 3 biological cell samples. **P < 0.01, ****P = 0.001, two-way ANOVA with Dunnett’s multiple comparisons test (e) or with Tukey’s multiple comparison test (f). Boxes are as in Fig. 2e. See Source Data for exact P values. Unless stated otherwise, data are mean ± s.d.
