Extended Data Fig. 7: Growth defect due to loss of SRC-3 or PFKFB4 is rescued by exogenous purines.
From: Metabolic enzyme PFKFB4 activates transcriptional coactivator SRC-3 to drive breast cancer
a, Expression of the metabolic enzymes encoded by TKT, XDH, AMPD1 and SRC-3 in MDA-MB-231 cells expressing control shRNA, SRC-3 shRNA or SRC-3 shRNA plus re-expression of shRNA-resistant wild-type SRC-3 protein (shSRC-3-21 + WT-SRC-3). n = 4 biological cell samples. *P < 0.05, **P < 0.01, ***P < 0.001, ****P = 0.0001, two-way ANOVA with Tukey’s multiple comparisons test. b, Relative proliferation of MDA-MB-231 cells expressing shRNA targeting SRC-3 (shSRC-3#01 and shSRC-3#02) or non-targeting control shRNA after treatment with siRNAs targeting luciferase (siLuc; as a control) or PFKFB4 under the conditions indicated. n = 6 samples from biologically independent experiments. ****P < 0.000001, two-way ANOVA with Tukey’s multiple comparisons test. c, MDA-MB231 cells stably expressing control shRNA, PFKFB4 shRNA or SRC-3 shRNA were fed with [U-13C]glucose for 48 h. Adenosine 13C-labelling from [U-13C]glucose is shown. n = 3 samples from biologically independent experiments. one-way ANOVA with Tukey’s multiple comparisons test. Boxes are as in Fig. 2e. Data are representative of three biologically independent experiments with similar results. See Source Data for exact P values. Unless stated otherwise, data are mean ± s.d.
