Extended Data Fig. 10: Drug response data.
From: Intra-tumour diversification in colorectal cancer at the single-cell level
Dose response data for seven drugs, tested on organoid clones from three individuals. Twenty-one concentrations were tested for each drug, ranging from 14.7 nm to 20 μM. Mean survival from two duplicate experiments is displayed in a heatmap. The concentration displayed in Fig. 4 is outlined with a black box in each panel. b, Reproducibility of drug response data. Each measurement was performed twice (technical replicate) and each experiment was performed in duplicate (biological replicate). For each biological or technical replicate the area under the curve (AUC) is shown. c, Dose–response curves after 6 days of treatment with IWP2 (Wnt secretion inhibitor) for clonal tumour organoids derived from P1. RNF43 mutant clones are responsive, whereas RNF43 wild-type (WT) clones are resistant. Data points and error bars represent the mean and s.d. of four independent technical replicates from two independent experiments.
