Extended Data Table 2 Structural alignments of MYCBP2 tandem cysteine domain

From: Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity

  1. Representative tandem-cysteine-domain sequences (130) from various taxa were isolated and aligned as described above. In virtually all animals, a single MYCBP2 orthologue is the only tandem-cysteine-domain-containing gene. There is a relatively large subfamily of sequences found in ciliates and a few other protists. Typically, each of these organisms contains multiple genes of this family. These proteins are also shorter than the animal orthologues. The alignment was rendered with Boxshade. Residues that are invariant in 50% of sequences or conservatively substituted are shown on black and grey backgrounds, respectively. Finally, Cys and His residues involved in Zn coordination are indicated in blue, while the Cys and His residues involved in the ubiquitin relay are shown in red.
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