Extended Data Fig. 8: Behavioural specificity of MPOAGal projection stimulation.
From: Functional circuit architecture underlying parental behaviour
a, Channelrhodopsin-2 (ChR2) expression in MPOAGal neurons (97.7 ± 0.2% overlap, virgin females, n = 2). Scale bar, 50 μm. b–g, Effect of activating PAG (b, c), VTA (d, e) or MeA (f, g) projections on time spent in nest in virgin females and virgin males (b, n = 13 females and n = 10 males; d, n = 9 females and n = 10 males; f, n = 10 females and n = 10 males) and number of pup-directed sniffing bouts (c, n = 13 females and n = 10 males; e, n = 9 females and n = 10 males; g, n = 10 females and n = 10 males). h–m, Effect of activating PAG (h, i), VTA (j, k) or MeA (l, m) projections on locomotion velocity (h, n = 13 females and n = 10 males; j, n = 8 females and n = 10 males; l, n = 10 females and n = 10 males) and moved distance (i, k, m). n, q, s, Effect of inhibiting PAG (n, n = 10 females), VTA (q, n = 10 females) or MeA (s, n = 11 females) projections on pup interactions. o, t, Effect of inhibiting PAG (o, n = 10 females) or MeA (t, n = 11 females) projections on number of barrier crosses. p, r, Effect of inhibiting PAG (p, n = 10 females) or MeA (r, n = 11 females) projections on chemoinvestigation of a male intruder. u–w, Effect of inhibiting PAG (u), VTA (v) or MeA (w) projections on locomotion velocity and moved distance (n = 10, 10, 11, respectively). Two-tailed paired t-tests; c, *P = 0.0135; f, *P = 0.03; n, *P = 0.0413, q: *P = 0.0264.
