Extended Data Fig. 1: Putative functional roles of brain areas providing monosynaptic inputs into MPOAGal neurons.
From: Functional circuit architecture underlying parental behaviour
a, Comparison between MPOAGal input fractions in virgin males (n = 3) and virgin females (n = 3) after rabies tracing (see Fig. 1a). Sexually dimorphic inputs are highlighted. Two-tailed t-tests, supraoptic nucleus (SON), **P = 0.0041; posteriomedial amygdalo-hippocampal area (AHPM), ***P = 0.0007; medial septum (MS), *P = 0.0133. b, Comparison between MPOAGal input fractions after rabies tracing was initiated from the right (n = 3) or left (n = 3) hemisphere in virgin females. No significant differences were found (P > 0.05; two-tailed paired t-test). c, Comparison between rabies-injected (ipsilateral (ipsi)) and non-injected (contralateral (contra)) MPOA of a mother after parental behaviour. Activated (Fos+) rabies+ neurons are shown (top, arrowheads). Fos+ neuron numbers are not significantly different between hemispheres (bottom, P = 0.43, 95% confidence interval −4.176–1.843; two-tailed paired t-test; n = 6). d, MPOAGal neurons receive monosynaptic inputs from magnocellular SONAVP neurons (mothers, 72.7 ± 9.3% overlap, n = 3; virgin females, 77.4 ± 4.3%, n = 3; fathers, 83.3 ± 3.3%, n = 3) but rarely from SONOXT neurons (mothers, 4.6 ± 4.2% overlap, n = 2; virgin females, 4.5 ± 1.0%, n = 2; fathers, 2.8 ± 1.8%, n = 2). Data are mean ± s.e.m. Scale bars, 100 μm (c) and 50 μm (d).
