Extended Data Fig. 2: Representative negative-stain EM images, cryo-EM images, and 2D averages of the RTIC.
From: Architecture of an HIV-1 reverse transcriptase initiation complex
a, Representative negative-stain EM image of HIV RTIC reveals a mono-disperse sample that is free of aggregates. Approximately a dozen images were taken of each sample before cryo-EM grid preparation to ensure sample quality. b, Cryo-EM image of RTIC without β-OG. The long chains correspond to RNA from the complex with very few particles resembling the protein. Results are reproducible in the absence of β-OG (>10 samples tested). c, Cryo-EM image of RTIC with β-OG. Single particles corresponding to the complex appear similar to the negative-stain visualization. All 5,107 images used in both cryo-EM datasets have a similar appearance with slight differences in particle density. d, Representative 2D averages of RTIC complex from the cryo-EM data collected with β-OG. Both datasets exhibit very similar 2D classes.
