Extended Data Fig. 5: Mg2+ global map views and structure comparison.
From: Architecture of an HIV-1 reverse transcriptase initiation complex
a, Side and top views of the 8.2 Å global map at different density thresholds. The orientation of the peripheral vRNA and tRNA elements is within the variability seen among the different RTIC conformers. b, A model of the RTIC built into the Mg2+ density using the main text global RTIC model. vRNA and tRNA helices were treated as rigid bodies derived from main text model (see Extended Data Fig. 6 and Methods). c, Comparison of the global RTIC model RNA (grey) with the Mg2+ model RNA (coloured). All three regions of RNA structure (H1, H2, and tRNA) differ in the Mg2+ model, but are adequately described by rigid body movements of the RNA helical elements taken from the global RTIC model. Both H1 and H2 represent a substantial structural barrier to initiation. d, Partial accommodation of H1 into high monovalent salt classes 3, 4 and 7.
