Extended Data Fig. 6: Expression analyses in cells derived from mutant embryos.
From: RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6
a, qPCR analyses for Lgr4, Lgr5 and Lgr6 in NPCs derived from embryos of indicated genotypes. n = 4 biological replicates. b, qPCR analyses for Lgr4 and Lgr6 in iPS cells derived from NPCs of indicated genotypes. n = 3 biological replicates. c, qPCR analyses for Lgr4 and Lgr5 in SV40-immortalized dermal fibroblasts derived from embryos of indicated genotypes. n = 3 biological replicates. d, qPCR analyses for Rspo2, Rspo3 and Znrf3 in Lgr4/5/6 triple-knockout SV40-immortalized fibroblasts, transfected with indicated siRNAs. n = 3 biological replicates. Data are mean ± s.e.m. NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA test with Bonferroni’s correction.
