Extended Data Fig. 3: Mouse Lgr4/5/6 knock-in embryos do not recapitulate the Rspo2 and Rspo3 phenotypes.
From: RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6
a, Table indicating the proportion and numbers of analysed embryos. The different genotypes were obtained in Mendelian ratio (P > 0.05, χ2 test). b, Normal limbs of embryos with indicated genotypes including a Lgr4/5/6 triple-knockout embryo. All obtained embryos (see a) had a similiar phenotype. FL, forelimbs; HL, hindlimbs; L, left; R, right. Scale bar, 1 mm. c, Normal lungs of embryos with indicated genotypes including a Lgr4/5/6 triple-knockout embryo. Comparable lung length relative to body length is indicated. Scale bar, 1 mm. All obtained embryos (see a) had a similiar phenotype. d, H&E staining of coronal sections of the heads through the oral cavity. Scale bar, 1 mm. Close-ups of the palatal shelves illustrate the cleft palate present in the Lgr5/6 double-knockout (n = 2) and Lgr4/5/6 triple-knockout (n = 2) embryos (indicated by black arrow heads). e, Properly vascularized placenta of a Lgr4/5/6 triple-knockout embryo compared to a Lgr6-knockout embryo. All obtained embryos (see a) had a similiar phenotype. Scale bar, 1 mm.
