Extended Data Fig. 4: Mouse Lgr4, Lgr5 and Lgr6 knock-in embryos recapitulate the knockout phenotypes.
From: RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6
a, Illustration of the GFP knock-in in exon 1 of the Lgr4, Lgr5 and Lgr6 genes, which cause loss-of-function mutations. b, Liver weight in single Lgr4−/− (n = 2) and triple Lgr4/5/6−/− (n = 2) compared to wild-type (n = 3) E14.5 embryos. Data are mean ± s.e.m. NS, not significant. *P < 0.05, one-way ANOVA test with Bonferroni’s correction. c, Lgr4−/− embryos (n = 4) have a smaller liver. Liver weight is indicated. Scale bar, 1 mm. d, Lgr4−/− embryos (n = 2) show female-to-male sex reversal. Blue arrowheads point to male-specific coelomic vessels. Genetic genders (XY or XX) are indicated. Scale bar, 0.1 mm. e, H&E staining of coronal sections of the heads through the oral cavity. Scale bar, 1 mm. Close-ups of the tongue illustrate the ankyloglossia phenotype (tongue attached to the mouth floor, black arrow heads) in Lgr5−/− embryos (n = 4), whereas the tongue is detached for other genotypes (white arrowheads).
