Extended Data Fig. 5: Mouse Lgr4, Lgr5 and Lgr6 knock-in cause loss-of-function mutations.
From: RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6
a, b, qPCR analyses for Lgr4, Lgr5 and Lgr6 expression in E14.5 limbs (a) and lungs (b) of wild-type, heterozygous and homozygous mutant embryos for the respective genes. c, d, qPCR analyses for Lgr4 and Lgr5 in embryonic intestine (c) and embryonic liver (d) of embryos with indicated genotype. e, qPCR analyses for Lgr6 in embryonic and adult skin of wild-type versus homozygous animals. n indicates number of embryos. Data are mean ± s.e.m. NS, not significant. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA test with Bonferroni’s correction or two-tailed unpaired t-test with Welch’s correction when less than three groups were compared (for Lgr6 qPCR analysis). f, Duplex RNAscope for the indicated gene (blue) and Rspo2 (pink) in transverse sections of wild-type E14.5 lungs. Strongly expressed genes are denoted in bold (summary on the right). Scale bars, 0.2 mm. Experiment repeated with three different wild-type embryos.
