Extended Data Fig. 9: Effect of SdeA substrate-binding mutations in vivo.
From: Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination
a, In vitro Rab33b ubiquitination assays with GFP–SdeAFL substrate-binding mutants purified from HEK293T cells. b, Expression and translocation of SdeA using wild-type and various mutant strains of Legionella. c, CFU fold change monitored in wild-type Legionella and ΔsidEs strain complemented with substrate ubiquitination defective mutant plasmids (n = 3 biological replicates). Data shown as mean ± s.e.m. d, Co-localization of Legionella-containing vacuoles and RTN4 networks in primary mouse macrophages. Experiments in a, b and d were repeated twice independently with similar results. For gel source data, see Supplementary Fig. 1.
