Extended Data Fig. 4: Apoptosis and autophagy analyses in kidneys and hearts of aged mice.
From: Disruption of the beclin 1–BCL2 autophagy regulatory complex promotes longevity in mice
a, b, Representative images and quantification of active caspase 3-positive cells in kidneys (a) and hearts (b) from Becn1+/+ (wild-type) and Becn1F121A/F121A (knock-in) animals. Two month-old wild-type and knock-in mouse kidneys and hearts (n = 6 per genotype) were analysed. For kidney analyses, aged (20-month-old) wild-type (n = 20) and knock-in (n = 26) mice were used. For heart analyses, aged (20-month-old) wild-type (n = 19) and knock-in (n = 26) mice were used. Scatter plot bars represent median ± interquartile ranges. P values were determined by two-sided Mann–Whitney test. c, d, Enlarged versions of the endogenous LC3 puncta (autophagosome) images shown in Fig. 3d (c) and Fig. 3h (d). Arrows denote representative LC3 puncta.
