Extended Data Fig. 1: E06-scFv expression and binding characteristics.
From: Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice
a, Simply blue staining of purified E06-scFv from HEK293 cell lysates from two experiments. b, Western blot with anti-Myc antibody of E06-scFv following purification on Ni-NTA agarose beads (representative of four independent experiments). c, Binding profile of purified E06-scFv using chemiluminescent ELISA. Binding data are mean ± s.e.m., using three independent samples, each determined in triplicate. d, Tissue distribution of the E06-scFv gene transcript in Ldlr−/−E06-scFv mice determined by qPCR. Data are mean ± s.e.m., determined from tissues of three Ldlr−/−E06-scFv mice. e, Competition immunoassays of Ldlr−/−E06-scFv plasma binding to plated OxLDL in the presence or absence of increasing amounts of indicated competitors. Results are the ratios of E06-scFv binding to OxLDL in the presence (B) or absence of a competitor (B0). AB1-2 is a T15 anti-idiotypic antibody; C16lysoPC, C16 lysophosphatidylcholine; DPPC, dipalmitoyl phosphatidylcholine. Data are triplicates of each point from one competition experiment, representative of four separate studies of similar nature. f, Accumulation of desmosterol and other indicated sterols in TGEM from indicated mice fed a HCD for 16 weeks. TGEM were isolated from three mice in each group and each set of macrophages divided into two separate aliquots for analysis in triplicate. Data are mean ± s.e.m. There were no differences between respective sterol pairs, P > 0.05 for all pairs.
