Extended Data Fig. 2: Expressed E06-scFv does not alter the levels of total IgM or IgM–E06 (detected by AB1-2) in transgenic mice.
From: Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice
Comparison of plasma IgM titres to indicated antigens of Ldlr−/−or Ldlr−/−E06-scFv mice at baseline or after four or seven months of HCD. Note significant increases in total IgM and IgMs against MDA-LDL and OxLDL at four and seven months compared to respective baseline titres (all values, P < 0.001) except at four months, at which time the total IgM of Ldlr−/−E06-scFv mice and E06 (detected by AB1-2) in both mouse groups were not different from their respective baselines (P > 0.05). Notably, there were no significant differences in any antibody titres between Ldlr−/− or Ldlr−/−E06-scFv mice at any time point, and in particular, note that endogenous IgM–E06 titres (detected by AB1-2 binding) were similar. As expected, the plasma from Rag1−/− and Rag−/−E06-scFv mice did not have any IgM.
