Extended Data Fig. 3: Plasma E06-scFv binds to atherosclerotic lesions and apoptotic thymocytes and is present in the aorta of Ldlr−/−E06-scFv mice.
From: Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice
a, Staining of atherosclerotic lesions of Watanabe heritable hyperlipidaemic (WHHL) rabbit aortas with E06-scFv plasma (left) and plasma from Ldlr−/− mice (right) (both at dilution of 1:20), visualized using a biotinylated anti-Myc monoclonal antibody and ABC-AP VectaStain kit. b, Deconvolution microscopy of E06-scFv plasma (1:20 dilution) binding to apoptotic but not normal cells. Blue, nuclei stained with Hoechst; green, FITC-labelled anti-His6-tag monoclonal antibody; red, annexin V–PE. c, Binding of E06-scFv plasma (1:20 dilution) to apoptotic thymocytes (7AAD+annexin V+) isolated by FACS analysis. d, Expression of E06-scFv in aortic lesion of a Ldlr−/−E06-scFv but not a Ldlr−/− mouse. Cross-sections at the aortic valve were stained with biotinylated anti-Myc monoclonal antibody to identify presence of E06-scFv in atherosclerotic lesion. Nuclei counterstained using haematoxylin QS (Original ×200). a–c, Representative of similar studies with five other plasma samples from each genotype. d, Representative of studies in three other aortic sections of each genotype.
