Extended Data Fig. 3: UV light is detrimental to exposed haematopoiesis.
From: Protection from UV light is an evolutionarily conserved feature of the haematopoietic niche
a, Experimental layout. b, Reduction in HSPCs in larvae with and without PTU treatment as assessed by cmyb in situ hybridization after UVC irradiation. n = 26, 26, 24 and 19 larvae in from left to right. χ2 test. c, Reduction in HSPCs in tyr−/− larvae and their pigmented siblings as assessed by cmyb in situ hybridization after UVB irradiation at a UV index of 20. n = 38, 33, 29 and 38 larvae from left to right. Results were not significant but note that the pigmented irradiated group seemed to retain more HSPCs in this experiment than in the preceding experiments with a higher UV index. d, Histology of the kidney marrow two days after irradiation. The yellow outline represents the kidney tubules, the red outline shows the aorta and the green outline indicates the haematopoietic marrow. Note the reduced area of the haematopoietic marrow in the non-pigmented, irradiated larvae (bottom right). Scale bars, 20 μm. e, Abundance of thrombocytes positive for Tg(CD41:GFP) two days after irradiation. Each data point represents the number of thrombocytes per vessel area in individual larvae. n = 8, 7, 7 and 7 larvae for treatment groups from left to right. Statistical significance was calculated using ANOVA with post hoc Bonferroni’s multiple comparison test, data are mean ± s.d. f, Schematic of the analysis of the number of thrombocytes that were positive for Tg(CD41:GFP). The boxed area represents the analysed area, in which the circulating cells were counted. The yellow outline represents the area of the vessel, which the number of circulating thrombocytes was normalized to.
