Extended Data Fig. 9: Effect of rapamycin treatment on the different PIK3CACAGG-CreER mouse models.
From: Targeted therapy in patients with PIK3CA-related overgrowth syndrome
a, Kaplan–Meier survival curves of PIK3CACAGG-CreER mice that received a single dose of 40 mg kg−1 tamoxifen and were treated with or without rapamycin after tamoxifen administration. b, Representative pictures of the liver of PIK3CACAGG-CreER mice treated with rapamycin 40 days after Cre induction. c, Morphology of livers and spleens from PIK3CAWT and PIK3CACAGG-CreER mice that were treated with or without rapamycin after Cre induction. Scale bars, 10 μm. d, Western blot and quantification of P-AKT (Ser473) and P-S6RP in the liver, heart and muscle, respectively, of PIK3CAWT and PIK3CACAGG-CreER mice treated with vehicle or rapamycin directly after Cre induction. e, PIK3CACAGG-CreER mice were treated with rapamycin one month after Cre induction with a single dose of 4 mg kg−1 tamoxifen and followed for one month. All data are shown as mean ± s.e.m. ANOVA followed by Tukey–Kramer test (two-tailed). PIK3CACAGG-CreER versus PIK3CAWT mice, ***P < 0.001. PIK3CACAGG-CreER mice treated with rapamycin compared with PIK3CACAGG-CreER mice treated with vehicle, ###P < 0.001.
