Extended Data Fig. 4: Senescence and BYL719.
From: Targeted therapy in patients with PIK3CA-related overgrowth syndrome
a, β-galactosidase staining in the liver, heart, spleen and kidney of PIK3CAWT and PIK3CACAGG-CreER mice with or without BYL719 and quantification of β-galactosidase-positive cells per field (n = 8 mice per group, 10 randomly selected fields, ×400). C+: positive control. Scale bars, 10 μm. b, p16 mRNA expression in liver, heart and spleen of PIK3CAWT and PIK3CACAGG-CreER mice treated with or without BYL719 (n = 8 mice per group). A.U., arbitrary unit. All data are shown as mean ± s.e.m. ANOVA followed by Tukey–Kramer test (two-tailed).
