Extended Data Fig. 10: Inhibition of SIK3 kinase activity reduces phosphorylation of AMPK substrates in sleep-deprived wild-type brains.
From: Quantitative phosphoproteomic analysis of the molecular substrates of sleep need
a–c, Volcano plots showing phosphorylation changes of all putative AMPK substrates in the SD6/RS3 (a), SD6/S6 (b) and time-course sleep-deprivation groups (c). Orange dotted lines, Q = 0.2. d, e, Volcano plots showing comparative analysis of whole brain phosphoproteome (d) and phosphorylation changes of all putative AMPK substrates (e) in the HG/vehicle (WT-SD) group. Orange dotted lines, Q = 0.2. f–h, Analysis of absolute EEG power spectra (f), relative EEG power spectra (g) and duration (h) of NREMS, REMS and wake states of sleep-deprived (ZT0–ZT6) wild-type (n = 16) mice injected with vehicle (Veh) or 8 mg/kg HG at ZT0 and ZT3. Multiple unpaired t-test (P value) followed by FDR (Q value) analysis (a–e). Mean ± s.e.m., two-way ANOVA with Sidak’s test (f–h). *(black), P < 0.05; *(cyan), P < 0.01; *(red), P < 0.001; ns, P > 0.05.
