Extended Data Fig. 3: Analysis of sleep phenotype and signalling changes after food-and-water deprivation in the baseline and sleep deprivation conditions.
From: Quantitative phosphoproteomic analysis of the molecular substrates of sleep need
a–c, Analysis of circadian (a) and mean (b) absolute NREMS delta power, and duration (c) of NREMS, REMS and wake states, of wild-type mice (n = 8) without (sham) or with 6 h of food-and-water deprivation (FD 6 h). d, Quantitative analysis of immunoblots with six phosphorylation-motif antibodies using whole brain lysates of sham and 6-h food-and-water deprived mice (n = 8) collected at ZT6. e–g, Analysis of circadian (e) and mean (f) absolute NREMS delta power, and duration (g) of NREMS, REMS and wake states, of wild-type mice (n = 11) without (SD + sham) or with 6-h food-and-water deprivation during 6-h sleep deprivation (SD + FD 6 h). h, Quantitative analysis of immunoblots with six phosphorylation-motif antibodies using whole brain lysates of SD + sham and SD + FD mice (n = 6) collected at ZT6. Mean ± s.e.m., two-way ANOVA, Sidak’s test (a, c, e, g); Paired t-test, two-tailed (b, f); Mean ± s.d., two-way ANOVA, Fisher’s LSD test (d, h). *(black), P < 0.05; ns, P > 0.05.
