Extended Data Fig. 4: A conserved linker between Ubl and UPD.

Sequence alignment of parkin, with domains coloured corresponding to 5N2W⁶ as in Extended Data Fig. 1. Phosphate binding pockets are labelled. The linker region between Ubl and UPD (amino acids 76–143) contains two strings of highly conserved residues. Residues upstream and downstream of the conserved region are unconserved both in sequence and linker length. Tsparkin shows the smallest number of residues in the linker (upstream, 25 amino acids in human parkin, 18 amino acids in Tsparkin; downstream, 18 amino acids in human parkin, 11 amino acids in Tsparkin). See also Extended Data Fig. 8d.