Extended Data Fig. 1: Full alignment of heliorhodopsin and type-1 rhodopsin.
From: A distinct abundant group of microbial rhodopsins discovered using functional metagenomics
a, Multiple amino acid alignment of different heliorhodopsins. Positions 23, 80, 104 and 107 (heliorhodopsin 48C12 numbering) are marked with black arrows, as well as position 237 (grey arrow) and lysine in position 241 (red arrow). b, Multiple amino acid alignment of heliorhodopsin (48C12) with green absorbing proteorhodopsin (GPR), bacteriorhodopsin (BR), sensory rhodopsin I from Halobacterium salinarum (HsSRI) and Salinibacter ruber (SrSRI), sensory rhodopsin II from H. salinarum (HsSRII) and Natronomonas pharaonis (NpSRII), Anabaena sensory rhodopsin (ASR), xenorhodopsin from Parvularcula oceani (PoXeR), halorhodopsin from H. salinarum (HsHR), eubacterial chloride-pump rhodopsin from Nonlabens marinus (NmClR), sodium-pump rhodopsin from Krokinobacter eikastus (KR2) (K. eikastus is also known as Dokdonia eikasta), anion channelrhodopsin from Guillardia theta (GtACR1) and cation channelrhodopsin2 from Chlamydomonas reinhardtii (C-terminal side omitted, ChR2 ΔC-term). Positions 85, 89 and 96 (bacteriorhodopsin numbering) are marked with black arrows, as well as position 212 (grey arrow) and lysine in position 216 (red arrow).
