Extended Data Fig. 2: Relationship and depth distribution of heliorhodopsins and type-1 rhodopsins.
From: A distinct abundant group of microbial rhodopsins discovered using functional metagenomics
a, Clustering analysis separates heliorhodopsins (purple leaves in dendrogram tree) from type-1 rhodopsins (grey leaves in dendrogram tree) based on per cent identity obtained using protein–protein blast v.2.7.157. The hierarchical clustering was performed using the clustermap function from the Python package seaborn, with default parameters for metric and linkage. The code used to generate the figure is available as a Jupyter notebook at https://github.com/BejaLab/heliorhodopsin. b, Detailed phylogenetic relationships within the heliorhodopsin family. White circles represent bootstrap values of >80%. The scale bar indicates the average number of amino acid substitutions per site. Coloured circles indicate heliorhodopsins expressed in this study. c, Depth profiles of the relative abundance of type-1 rhodopsins and heliorhodopsins from marine metagenomes collected during the Tara Oceans expedition.
