Extended Data Fig. 6: Comparison of active A1R with active A2AR (PDB code 5G53) or agonist-bound ‘intermediate’ state A2AR (PDB code 2YDV).
From: Structure of the adenosine-bound human adenosine A1 receptor–Gi complex
a–j, Side views (a, f), extracellular views (b, g) and cytoplasmic views (c, h) of the active ADO–A1R–Gi2 structure (blue) compared to the active NECA–A2AR–mini-Gs structure (a–e) or ‘intermediate’ NECA–A2AR structure (orange) (f–j). d, i, Orthosteric binding site of the active A1R–Gi2 complex with ADO (purple ball and sticks) or A2AR with NECA (orange ball and sticks). ‘Toggle switch’ residue W6.48 and residues within 4 Å of ADO are labelled and shown as sticks. N, O and S atoms are coloured in blue, red and yellow, respectively. Dashed lines represent hydrogen bonds. e, j, Conserved class A GPCR motifs important for receptor activation (DRY motif, purple; NPXXY motif, blue; PIF motif, green).
