Extended Data Fig. 7: WASP and ARP2/3 mediate DSB repair by homology-directed mechanisms

a, Representative FACS plots of GFP⁺ cells in the HDR (DR-GFP) assay. b, Western blot shows expression of WASP and N-WASP following WASP siRNA knockdown in whole-cell lysates (single experiment). WASP siRNA 1: 5′-GAGUGGCUGAGUUACUUGC-3′. c, Representative FACS plots of GFP⁺ cells in the HDR (DR-GFP) assay in WASP and mock-depleted cells. d, Summary of DR-GFP assay with WASP depletion. P calculated by one-way ANOVA with multiple comparisons; data shown as mean and s.e.m; n = 3 independent experiments. HDR efficiency in the presence of DMSO or wiskostatin (Fig. 5a) shown for comparison. e, Representative FACS plots of GFP⁺ cells in the SSA (SA-GFP) assay. f, Representative FACS plots of GFP⁺ cells in the MMEJ (EJ2-GFP) assay. g, Representative FACS plots of GFP⁺ cells in the NHEJ (EJ5-GFP) assay. h, Summary of DR-GFP, SA-GFP, and EJ5-GFP assays with CK-689. P calculated by two-way ANOVA with multiple comparisons; data shown as mean and s.d.; n = 3 independent experiments. NS, not significant.