Extended Data Fig. 8: Actin nucleation regulates HDR in the nucleus and does not require formin-2 activity.
From: Nuclear ARP2/3 drives DNA break clustering for homology-directed repair
a, Western blot shows expression of mCherry-tagged actin constructs in U2OS whole-cell lysates (single experiment). b, Summary of DR-GFP (HDR) assay. P calculated by one-way ANOVA with multiple comparisons; data shown as mean and s.e.m; n = 6 independent experiments. c, Western blot shows knockdown of formin-2 following 48 h transfection of mock or sequence-specific siRNA oligos (single experiment). Formin-2 siRNA #2: 5′-CGUGUAAUCAGAAUGCCCA-3′. d, Summary of DR-GFP assay. P calculated by one-way ANOVA with multiple comparisons; data shown as mean and s.e.m; n = 3 independent experiments. e, Mean cell survival after 48 h exposure to increasing concentrations of the formin inhibitor SMIFH2 (n = 3 independent experiments). f, Summary of DR-GFP assay. P calculated by Student’s two-tailed t-test; data shown as mean and s.e.m; n = 3 independent experiments.
