Extended Data Fig. 9: B lymphocytes derived from patients with WAS exhibit reduced DSB end-resection.
From: Nuclear ARP2/3 drives DNA break clustering for homology-directed repair
a, Representative cell cycle distribution of CB33 and RD lymphocytes derived from healthy individuals following DMSO treatment (left). The percentage of RPA-positive S-phase cells following DMSO treatment was measured by flow cytometry (right). b, Representative cell cycle distribution of lymphocytes bearing the V75M mutation in the WAS gene or a G>A transition at position 5 in intron 6 of the WAS gene (IVS6+5G>A) following DMSO treatment (left). The percentage of RPA-positive S-phase cells following DMSO treatment was measured by flow cytometry (right). c, Representative cell cycle distribution of CB33 and RD lymphocytes following CPT treatment (left). The percentage of RPA-positive S-phase cells following CPT treatment was measured by flow cytometry (right). d, The cell cycle distribution of WAS V75M or WAS IVS6+5G>A lymphocytes following CPT treatment (left). The percentage of RPA-positive S-phase cells following CPT treatment was measured by flow cytometry (right). For all panels, n = 4 independent experiments.
