Extended Data Fig. 6: vtc4⁺ and rpl3402⁺ are critical for inheritance of silencing at the endogenous ade6⁺ locus.

a, Schematic of the siRNA driver cen::ade6⁺ locus on chromosome 1 (upper) and the endogenous ade6⁺ locus (lower) in which the vt4⁺ and rpl3402⁺ genes were replaced with the ura4⁺ gene (vtc4Δ rpl3402∆::ura4⁺). b, Frequency of silencing establishment at endogenous ade6⁺ in cen::ade6⁺ leo1∆ vtc4Δ rpl3402∆ cells. Experiments were repeated twice. c, cen::ade6⁺ leo1∆ vtc4Δ rpl3402∆ ade6⁺ OFF cells were crossed to cen⁺ leo1⁺ ade6^BC+ ON cells and RSA was performed to test the epigenetic maintenance of the OFF state in the absence of the cen::ade6⁺ siRNA driver and the leo1∆ enabling mutation. The number of progeny matching each genotype and phenotype are shown. 80 white and 80 red progeny were genotyped. Results reflect two independent crosses.