Extended Data Fig. 8: Heritable silencing of KanR-ade6⁺ transgenes correlates with local secondary siRNA generation.

a–c, Zoomed-in (upper) or zoomed-out (lower) sRNA-seq reads mapping to the indicated KanR-ade6⁺ transgenes in cen⁺ mlo3⁺ leo1⁺ meiotic progeny of the crosses shown in Extended Data Fig. 7e–g. In c, two different red clones are shown, corresponding to clone 1 (upper) and clone 2 (lower) in Extended Data Fig. 7i. In these clones, the magnitude of locally hopped siRNAs (not mapping to KanR-ade6⁺) correlates with the magnitude and efficiency of inherited silencing. d, Zoomed-in view of sRNA-seq reads mapping to the endogenous ade6⁺ locus. Sequencing was performed once, but for meu10::KanR-ade6⁺ OFF, which represented a strong silent epiallele, small RNAs from two independent clones (1 and 2) were analysed.