Extended Data Fig. 2: The acquired ade6⁺ silent allele is stable in the absence of the cen::ade6⁺ siRNA trigger and the mlo3∆ enabling mutation.

a, ade6⁺ OFF progeny of the cross in Fig. 2b with the indicated genotypes were plated on low adenine medium. See Fig. 2c for related results. b–e, Biological replicates of the crosses shown in Fig. 2d–g. cen::ade6⁺ mlo3∆ ade6⁺ OFF cells were crossed to cen⁺ mlo3⁺ ade6^BC+ ON cells with deletions of key RNAi components (b–d) or H3K9 methyltransferase Clr4 (e) and RSA was performed. All ade6⁺ OFF progeny were RNAi⁺ and clr4⁺. Bars indicate number of ade6⁺ OFF meiotic progeny for each genotype.