Extended Data Fig. 7: Normal metabolic function of naive P2rx7−/− P14 CD8+ T cells, while pharmacological inhibition of P2RX7 compromises aerobic glycolysis of in vitro-activated CD8+ T cells.
From: The purinergic receptor P2RX7 directs metabolic fitness of long-lived memory CD8+ T cells
a–c, Naive wild-type and P2rx7−/− P14 cells were isolated and evaluated for different metabolism parameters (data from three independent experiments, n = 6 total). d, e, Human CD8+ T cells were activated in vitro, with the P2RX7 inhibitor A-438079 or vehicle control added 20 h after initiation of the culture, and assessed at 72 h for ECAR (d) or for viability of cells cultured in parallel under the same conditions as those used for extracellular flux assays (e). d, e, Data are from three independent experiments, n = 4–6 from all experiments. All data shown as mean ± s.e.m.; b, c, e, Two-tailed Student’s t-test.
