Extended Data Fig. 5: Defective metabolism of IL-15-polarized CD8+ T cells in the absence of P2RX7.
From: The purinergic receptor P2RX7 directs metabolic fitness of long-lived memory CD8+ T cells
a, Viability of wild-type and P2rx7−/− P14 cells maintained under culture conditions used for extracellular flux assays for 4 h (data from two independent experiments, n = 11 from all experiments). b–d, Wild-type or P2rx7−/− P14 cells were activated in vitro and subsequently polarized in IL-2 or IL-15 for 72 h and assayed for ECAR to measure aerobic glycolysis (b); uptake of TMRE to measure mitochondrial membrane potential (data normalized to MTG staining; c); and staining with MTG to determine total mitochondrial mass (d). Data from three independent experiments, n = 5–6 from all experiments. e, Wild-type and P2rx7−/− P14 cells were activated in vitro for 72 h, then stimulated with IL-15 (or not) for 30 min and immediately assayed for pSTAT5 expression. Data are representative of two independent experiments (n = 6 total). f, Wild-type and P2rx7−/− P14 cells were activated in vitro for 72 h, then stimulated with IL-15 or IL-2 for 72 h and assayed for expression of CD62L. Data are representative of three independent experiments (n = 6 total). g, Numbers of viable wild-type and P2rx7−/− P14 cells following activation and subsequent culture in IL-15 or IL-2 for the indicated number of days. Values for wild-type and P2rx7−/− cells were not significantly different (P > 0.05). Data from three independent experiments (n = 3–6 total). a–d, g, Mean ± s.e.m.; a, c, d, g, two-tailed Student’s t-test; **P ≤ 0.01.
