Extended Data Fig. 6: In vivo effects of C-176 in wild-type mice.
From: Targeting STING with covalent small-molecule inhibitors
a, Labelling of endogenous STING immunoprecipitaed from splenocytes of mice treated with C-176-AL, visualized by in-gel fluorescence. One representative of n = 2. b, Plasma concentration profiles of C-176 after a single-dose intraperitoneal injection into wild-type mice (n = 2 mice per condition). Data are mean of technical replicates. c, Serum levels of type I IFNs and IL-6 from wild-type mice pretreated with C-176 or vehicle 4 h after injection with CMA (n = 3 mice per condition). d, Body weight of wild-type mice during two weeks of daily DMSO and C-176 injection. e–g, Mice from d were euthanized and blood samples were collected for measuring plasma levels of TNFα and IL-6 (e), blood cell counts (f), and liver and kidney parameters (g). Data are mean ± s.d. of n = 10 mice per condition (e–g). P values were calculated by one-way ANOVA (c) or two-tailed t-test (e–g). ns, not significant. For gel source data, see Supplementary Fig. 1. For source data, see Supplementary Table 1.
