Extended Data Fig. 7: Activity of C-178 and C-176 in TREX1-deficient cells and mice.
From: Targeting STING with covalent small-molecule inhibitors
a, Wild-type (n = 2) or Trex1−/− (n = 7) mouse embryonic fibroblasts were treated with DMSO or C-178 (2 μM) overnight. mRNA levels of Isg15 and Cxcl10 were measured by RT–qPCR. b, c, Control mice (wild type or Trex1+/− (each n = 3)) were treated with vehicle, and Trex1−/− mice were treated with C-176 (n = 5 mice) or vehicle (n = 4 mice) for 11 days. Serum type I IFN levels (b) were measured and histological analysis of the heart (c) was performed. Scale bars, 50 μm. d, Histological analysis of distinct organs from wild-type (all n = 4) or Trex1−/− mice treated for 3 months. Smooth muscle, n = 3 (C-176) or 5 (vehicle); tongue and stomach, n = 3 (C-176) or 6 (vehicle). Representative histological images are shown. Data are mean ± s.e.m. P values were calculated using two-tailed t-test (a) or one-way ANOVA (b–d). For source data, see Supplementary Table 1.
