Extended Data Fig. 8: C-170 and C-171 antagonize hsSTING.
From: Targeting STING with covalent small-molecule inhibitors
a, Structure of C-170 and C-171. b, IFNβ luciferase reporter measurements from HEK293T cells transfected with indicated constructs (C-170 and C-171, 0.02–2 μM) (n = 3). c, d, THP-1 cells were pretreated with C-170 (0.5 μM) and stimulated with cGAMP or triphosphate RNA. IFNB1 and TNF mRNA levels were assessed by RT–qPCR (n = 5) (c), and p-TBK1 was determined by immunoblot (d). e, [3H]-palmitate labelling of HEK293T cells that express Flag–hsSTING, treated with C-170 (1 μM) or DMSO. f, HEK293T cells that express Flag–hsSTING (wild-type hsSTING or hsSTING(C91S)) were treated with C-170 (1 μM), lysed and Flag–hsSTING was analysed by intact mass measurement (LC-MS). Deconvoluted electrospray ionization mass spectrum showing intact mass indicated hsSTING constructs and treatments, are shown. Data are mean ± s.e.m. P values were calculated using two-tailed t-test. NS, not significant. One representative of n = 3 (d) and n = 2 (e–f) independent experiments is shown. For gel source data, see Supplementary Fig. 1. For electrospray ionization spectra see Supplementary Information.
