Extended Data Fig. 2: Activity of C-178 against STING in distinct cells.
From: Targeting STING with covalent small-molecule inhibitors
a, b, mRNA expression levels of Tnf in BMDMs activated with cGAMP, cyclic di-GMP (cdG), dsDNA, CMA or LPS for 5 h, after pretreatment for 1 h with C-178 (0.5 μM) or DMSO (n = 3 biological replicates). c, Heat map of RNA sequencing analysis of BMDMs treated with DMSO or CMA, in the presence or absence of C-178. The top 50 upregulated genes in cells treated with DMSO versus cells treated with CMA, in the absence of C-178, are shown for all conditions. d, Scatter plot of the dimensions PC1 versus PC2. e, Levels of expression of IFNB1 mRNA in THP-1 and WI-38 cells pretreated with C-178 (0.25 μM) or DMSO and stimulated with cGAMP for 3 h (n = 2 biological replicates). f, HEK293T cells were transfected with the construct chimaeric 1 (C1: hsSTING (amino acids 1–138)–mmSTING (amino acids 138–378)) or the construct chimaeric 2 (C2: mmSTING (amino acids 1–137)–hsSTING (amino acids 139–379)), together with cdG-Syn and IFNβ luciferase reporter, and treated with C-178 (0.5 μM) (n = 3 biological replicates). Data are mean or mean ± s.e.m. P values were determined by two-tailed t-test.
