Extended Data Fig. 8: Western blot analysis of NfMCU expression in E. coli.
From: Cryo-EM structure of a fungal mitochondrial calcium uniporter
NfMCU, with its mitochondrial targeting sequence removed and replaced with a 6×His tag, was expressed in E. coli for functional analysis. Mouse anti-His antibodies (Qiagen) were used to detect NfMCU expression and mouse anti-RpoA targeting E. coli RNA polymerase alpha (Santa Cruz Biotechnology) was used for loading control. See Supplementary Fig. 1 for gel source data. The experiment was repeated three times independently with similar results.
