Extended Data Fig. 3: Cryo-EM analysis of NfMCU.
From: Cryo-EM structure of a fungal mitochondrial calcium uniporter
a, Representative phase-plate electron micrograph of the NfMCU saposin complex and 2,470 micrographs were used for structure determination. b, Representative 2D class averages of the NfMCU saposin complex. c, Flowchart of data processing to obtain the 3.8 Å cryo-EM map of the NfMCU saposin complex. Details can be found in the Image processing section of the Methods. d, Local resolution of NfMCU estimated with RELION2.0. e, FSC curves for the refined model versus the summed 3.8 Å map (black curve), the refined model versus half map 1 (red curve), and the refined model versus half map 2 that was not used for refinement (green curve). f, The gold-standard FSC curve for the cryo-EM map.
