Extended Data Fig. 8: ALL cell invasion along laminin matrices is regulated by PI3K signalling and α6 integrin.
From: Leukaemia hijacks a neural mechanism to invade the central nervous system
a, Primarly ALL and Nalm-6 in vitro invasion towards human CSF along collagen, collagen and laminin, or collagen and fibronectin matrices. Data are mean ± s.e.m.; ANOVA with Tukey, n = 3 biologically independent experiments; primary ALL: P = 0.8937 (collagen versus 0.001 mg ml−1 laminin), P = 0.0020 (collagen versus 0.005 mg ml−1 laminin), P < 0.0001 (collagen versus 0.01 mg ml−1 laminin), P = 0.0604 (0.01 mg ml−4 laminin versus fibronectin); Nalm-6: P = 0.0012 (collagen versus 0.01 mg ml−1 laminin), P = 0.0150 (0.01 mg ml−1 laminin versus fibronectin). b, Comparative in vitro invasion of low α6 integrin-expressing ALL cells versus high α6 integrin-expressing ALL cells towards human CSF along laminin matrices and effects of PI3Kδ inhibition or α6 integrin blockade. Data are mean ± s.e.m.; ANOVA with Tukey, n = 3 biologically independent experiments; REH: P > 0.9999 (collagen versus laminin), P = 0.6967 (collagen and laminin versus collagen and laminin and GS-649443), P > 0.9999 (collagen and laminin versus collagen and laminin and anti-integrin-α6); primary ALL: P = 0.9758 (collagen versus laminin), P = 0.9974 (collagen and laminin versus collagen and laminin and GS-649443), P = 0.9993 (collagen and laminin versus collagen and laminin and anti-integrin-α6); RCH: P = 0.2446 (collagen versus laminin), P = 0.0079 (collagen and laminin versus collagen and laminin and GS-649443), P = 0.2549 (collagen and laminin versus collagen and laminin and anti-integrin-α6); SUP-B15: P = 0.0122 (collagen versus laminin), P = 0.0080 (collagen and laminin versus collagen and laminin and GS-649443), P = 0.0112 (collagen and laminin versus collagen and laminin and anti-integrin-α6). *P < 0.05, **P < 0.01, ***P < 0.001.
