Extended Data Fig. 7: Analysis of Tas2r expression in single Flare25 and Flare25;Aire^−/− thymic tuft cells.

a, Intracellular DCLK1 in C57BL/6 control and Aire^−/− mTECs (CD11c⁻CD45⁻EPCAM⁺). n = 5 mice; three independent experiments. b, Histogram showing the number of Tas2r family members expressed in each individual thymic tuft cell. Note that the distribution is similar between wild-type and Aire^−/− cells. c, Correlation plot of Tas2r genes in single Flare25;Aire^−/− tuft cells as analysed in Fig. 3c. Colour and circle size represent pairwise correlation values. d, Empirical cumulative distribution function plot of pairwise gene expression correlation of single Flare25;Aire^−/− tuft cells. Red points represent Tas2r genes that were significantly highly correlated compared to the background gene set (P < 0.05). e, List of correlated Tas2r gene pairs from d and their corresponding empirical P values. f, Representative confocal maximum projections (25 μm) of immunofluorescence analysis of C57BL/6 control and Aire^−/− thymi stained for KRT5 (red) and KRT10 (green). Scale bars, 100 μm. n = 3 mice; two independent experiments. f, Analysis of DCLK1⁺ cells in Hipk2^fl/fl controls or Foxn1^creHipk2^fl/fl (mTEC Hipk2^−/−) thymus gated on CD45⁻CDR1⁻EPCAM⁺ mTECs as quantified in Fig. 3h. n = 6 mice; three independent experiments.